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| Registros recuperados : 22 | |
| 5. |  | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPAMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, p. 2-19, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPOMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, 2015. 19 p.| Biblioteca(s): Embrapa Soja. |
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| 7. | | REIS, R. R.; NAKAYAMA, T.; MARIN, S. R. R.; MARINHO, J. P.; MARCOLINO-GOMES, J.; MERTZ-HENNING, L. M.; MOLINARI, H. B. C.; NEPOMUCENO, A. L. Engenharia genética de precisão para tolerância a seca em soja. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 8. | | GOMES, J. M.; NAKAYAMA, T. J.; REIS, R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; MOLINARI, H. B. C.; HARMON, F.; NEPOMUCENO, A. L. Gene expression and functional analysis of soybean genes with diurnal oscillation during drought stress. In: PLANT & ANIMAL GENOME XXIII, 2015, San Diego. PO 203| Biblioteca(s): Embrapa Soja. |
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| 9. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; HARMONAND, F. G.; NEPOMUCENO, A. L. Gene expression networks and cis-elements combinatorial models in soybean: circadian clock and drought responses. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 45-46.| Biblioteca(s): Embrapa Soja. |
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| 10. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; HENNING, L. M. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic Arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Biblioteca(s): Embrapa Soja. |
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| 12. | | MARTINS, P. K.; NAKAYAMA, T. J.; RIBEIRO, A. P.; DIAS, B. B. A.; NEPOMUCENO, A. L.; HARMON, F. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis floral-dip: a simple and rapid Agrobacterium-mediated transformation method. Biotechnology Reports, v. 6, p. 61-63, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 13. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; MARCOLINO, J.; MARCELINO, F. C.; SILVA, F. R. da; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L.; NEUMAIER, N. Transcriptome analysis of soybean (Glycine max L. Merrill) root submited to hypoxic condition. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 154-155.| Biblioteca(s): Embrapa Soja. |
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| 14. | | GOMES, J. M.; RODRIGUES, F. A.; PAGLIARINI, R. F.; BENDIX, C.; NAKAYAMA, T. J.; CELAYA, B.; MOLINARI, H. B. C.; OLIVEIRA, M. C. N. de; HARMON, F. G.; NEPOMUCENO, A. Diurnal oscillations of soybean circadian clock and drought responsive genes. Plos One, v. 9, n. 1, p. 1-13, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI; BENDIX, C.; NAKAYAMA, T. J.; CELAYA, B.; MOLINARI, H. B. C.; OLIVEIRA, M. C. N. de; HARMON, F. G.; NEPOMUCENO, A. L. Diurnal oscillations of soybean circadian clock and drought responsive genes. Plos One, v. 9, n. 1, e86402, Jan. 2014. 13 p.| Biblioteca(s): Embrapa Soja. |
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| 16. | | RODRIGUES, F. A.; MARCOLINO, J.; ENGELS, C.; NAKAYAMA, T. J.; FUGANTI, R.; MARIN, S. R.; FARIAS, J. R. B.; NEUMAIER, N.; MARCELINO, F. C.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L. Prospecting soybean (Glycine max L. Merrill) genes expressed under drought conditions. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia. Charles Darwin: a origem das espécies: o livro que transformou a humanidade. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 92.| Biblioteca(s): Embrapa Soja. |
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| 17. | | RODRIGUES, F. A.; MARCOLINO, J.; NAKAYAMA, T. J.; FUGANTI, R.; ENGELS, C.; MARIN, S. R. R.; FARIAS, J. R. B.; NEUMAIER, N.; MARCELINO, F. C.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L. Prospecting soybean (Glycine Max L. Merrill) genes expressed under drought conditions. In: INTERNATIONAL CONFERENCE ON INTEGRATED APPROACHES TO IMPROVE CROP PRODUCTION UNDER DROUGHT-PRONE ENVIRONMENTS, 3., 2009, Shangai. Abstracts... Shangai: Shangai Academy of Agricultural Sciences: Shangai Agrobiological Gene Center, 2009. p. 178, ref. P 5.35. INTERDROUGHT 2009.| Biblioteca(s): Embrapa Soja. |
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| 18. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Soja. |
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| 19. | | MARCOLINO, J.; RODRIGUES, F. A.; AMARAL, L. C.; NAKAYAMA, T. J.; PEREIRA, R. M.; NASCIMENTO, L. C. do; BINNECK, E.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; ABDELNOOR, R. V.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Transcription factors expressed in soybean roots in response to water déficit. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 193-194.| Biblioteca(s): Embrapa Soja. |
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| 20. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. Plos One, v. 10, n. 9, e0139051, 2015. 16 p.| Biblioteca(s): Embrapa Agroenergia; Embrapa Soja. |
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| Registros recuperados : 22 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Soja. |
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Data corrente: |
20/02/2014 |
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Data da última atualização: |
05/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; CNPSo; NORMAN NEUMAIER, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; UFV; ANA CLAUDIA BARNECHE DE OLIVEIRA, CPACT; BEATRIZ MARTI EMYGDIO, CPACT; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014. |
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DOI: |
DOI: 10.4238/2014.February.13.4 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants. |
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Palavras-Chave: |
Endogenous genes; Flooding; Housekeeping genes; Internal control genes. |
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Thesaurus NAL: |
gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97888/1/nakayama.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97827/1/Ana-Claudia.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112937/1/Reference-genes-soybean-..hypoxic-conditions-Incluido.pdf
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Marc: |
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100 1 $aNAKAYAMA, T. J.
245 $aReference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions.$h[electronic resource]
260 $c2014
520 $aQuantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants.
650 $agene expression
653 $aEndogenous genes
653 $aFlooding
653 $aHousekeeping genes
653 $aInternal control genes
700 1 $aRODRIGUES, F. A.
700 1 $aNEUMAIER, N.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aFARIAS, J. R. B.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aBORÉM, A.
700 1 $aOLIVEIRA, A. C. B. de
700 1 $aEMYGDIO, B. M.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 13, n. 1, p. 860-871, 2014.
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